Construct: shRNA TRCN0000103146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015819.1-43s1c1
DNA Barcode:
GCTCTTCCTATTTGCGGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hs6st2 (50786)
Gene Description:
heparan sulfate 6-O-sulfotransferase 2
Transcript:
RefSeq NM_015819.1 (NON-CURRENT)
Match location:
Position 43 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTCCTATTTGCGGTGAT
Hairpin Sequence:
5'-CCGG-GCTCTTCCTATTTGCGGTGAT-CTCGAG-ATCACCGCAAATAGGAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTCCTATTTGCGGTGATCTCGAGATCACCGCAAATAGGAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTCCTATTTGCGGTGATCTCGAGATCACCGCAAATAGGAAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000317181