Construct: shRNA TRCN0000103180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013837.1-1594s1c1
DNA Barcode:
CCAGTCTTTAATTTCAAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpst1 (22021)
Gene Description:
protein-tyrosine sulfotransferase 1
Transcript:
RefSeq NM_013837.1 (NON-CURRENT)
Match location:
Position 1594 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTTTAATTTCAAGGAA
Hairpin Sequence:
5'-CCGG-CCAGTCTTTAATTTCAAGGAA-CTCGAG-TTCCTTGAAATTAAAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTTTAATTTCAAGGAACTCGAGTTCCTTGAAATTAAAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTTTAATTTCAAGGAACTCGAGTTCCTTGAAATTAAAGACTGG-3'

Other clones with same target sequence:

(none)