Construct: shRNA TRCN0000103203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016803.2-1711s1c1
DNA Barcode:
GATGTCTACTCCACTCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chst3 (53374)
Gene Description:
carbohydrate (chondroitin 6/keratan) sulfotransferase 3
Transcript:
RefSeq NM_016803.2 (NON-CURRENT)
Match location:
Position 1711 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTCTACTCCACTCAGAAA
Hairpin Sequence:
5'-CCGG-GATGTCTACTCCACTCAGAAA-CTCGAG-TTTCTGAGTGGAGTAGACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTCTACTCCACTCAGAAACTCGAGTTTCTGAGTGGAGTAGACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTCTACTCCACTCAGAAACTCGAGTTTCTGAGTGGAGTAGACATC-3'

Other clones with same target sequence:

(none)