Construct: shRNA TRCN0000103226

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015820.1-1260s1c1
DNA Barcode:
GCATCCAACGTGGACATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hs6st3 (50787)
Gene Description:
heparan sulfate 6-O-sulfotransferase 3
Transcript:
RefSeq NM_015820.1 (NON-CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103226)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCAACGTGGACATCAAT
Hairpin Sequence:
5'-CCGG-GCATCCAACGTGGACATCAAT-CTCGAG-ATTGATGTCCACGTTGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCAACGTGGACATCAATCTCGAGATTGATGTCCACGTTGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCAACGTGGACATCAATCTCGAGATTGATGTCCACGTTGGATGC-3'

Other clones with same target sequence:

(none)