Construct: shRNA TRCN0000103288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175467.1-911s1c1
DNA Barcode:
GCAATGAATATCCCTGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sptlc3 (228677)
Gene Description:
serine palmitoyltransferase, long chain base subunit 3
Transcript:
RefSeq NM_175467.1 (NON-CURRENT)
Match location:
Position 911 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGAATATCCCTGTATTT
Hairpin Sequence:
5'-CCGG-GCAATGAATATCCCTGTATTT-CTCGAG-AAATACAGGGATATTCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGAATATCCCTGTATTTCTCGAGAAATACAGGGATATTCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGAATATCCCTGTATTTCTCGAGAAATACAGGGATATTCATTGC-3'

Other clones with same target sequence:

(none)