Construct: shRNA TRCN0000103289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175467.1-1384s1c1
DNA Barcode:
GCAGTCACATAGCACTACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sptlc3 (228677)
Gene Description:
serine palmitoyltransferase, long chain base subunit 3
Transcript:
RefSeq NM_175467.1 (NON-CURRENT)
Match location:
Position 1384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCACATAGCACTACTTA
Hairpin Sequence:
5'-CCGG-GCAGTCACATAGCACTACTTA-CTCGAG-TAAGTAGTGCTATGTGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCACATAGCACTACTTACTCGAGTAAGTAGTGCTATGTGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCACATAGCACTACTTACTCGAGTAAGTAGTGCTATGTGACTGC-3'

Other clones with same target sequence:

(none)