Construct: shRNA TRCN0000103331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021439.1-1075s1c1
DNA Barcode:
CGATGTCAAGTTCGAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chst11 (58250)
Gene Description:
carbohydrate sulfotransferase 11
Transcript:
RefSeq NM_021439.1 (NON-CURRENT)
Match location:
Position 1075 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGTCAAGTTCGAGGAGTT
Hairpin Sequence:
5'-CCGG-CGATGTCAAGTTCGAGGAGTT-CTCGAG-AACTCCTCGAACTTGACATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGTCAAGTTCGAGGAGTTCTCGAGAACTCCTCGAACTTGACATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGTCAAGTTCGAGGAGTTCTCGAGAACTCCTCGAACTTGACATCG-3'

Other clones with same target sequence:

(none)