Construct: shRNA TRCN0000103335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026764.2-1030s1c1
DNA Barcode:
GCTGTGCTGTTGTGAAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gstm4 (14865)
Gene Description:
glutathione S-transferase, mu 4
Transcript:
RefSeq NM_026764.2 (NON-CURRENT)
Match location:
Position 1030 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGCTGTTGTGAAGAGTT
Hairpin Sequence:
5'-CCGG-GCTGTGCTGTTGTGAAGAGTT-CTCGAG-AACTCTTCACAACAGCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGCTGTTGTGAAGAGTTCTCGAGAACTCTTCACAACAGCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGCTGTTGTGAAGAGTTCTCGAGAACTCTTCACAACAGCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000302883