Construct: shRNA TRCN0000103350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013873.3-1452s1c1
DNA Barcode:
CGGAGATCAAAGAACGGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult4a1 (29859)
Gene Description:
sulfotransferase family 4A, member 1
Transcript:
RefSeq NM_013873.3 (CURRENT)
Match location:
Position 1452 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGATCAAAGAACGGGTTT
Hairpin Sequence:
5'-CCGG-CGGAGATCAAAGAACGGGTTT-CTCGAG-AAACCCGTTCTTTGATCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGATCAAAGAACGGGTTTCTCGAGAAACCCGTTCTTTGATCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGATCAAAGAACGGGTTTCTCGAGAAACCCGTTCTTTGATCTCCG-3'

Other clones with same target sequence:

(none)