Construct: shRNA TRCN0000103352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013873.3-374s1c1
DNA Barcode:
CCCTGATGAAATCGGCCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult4a1 (29859)
Gene Description:
sulfotransferase family 4A, member 1
Transcript:
RefSeq NM_013873.3 (CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATGAAATCGGCCTGAT
Hairpin Sequence:
5'-CCGG-CCCTGATGAAATCGGCCTGAT-CTCGAG-ATCAGGCCGATTTCATCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATGAAATCGGCCTGATCTCGAGATCAGGCCGATTTCATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATGAAATCGGCCTGATCTCGAGATCAGGCCGATTTCATCAGGG-3'

Other clones with same target sequence:

(none)