Construct: shRNA TRCN0000103391

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145935.2-799s1c1
DNA Barcode:
CCATTGATCAAGACAAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glyat (107146)
Gene Description:
glycine-N-acyltransferase
Transcript:
RefSeq NM_145935.2 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103391)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGATCAAGACAAGTTTA
Hairpin Sequence:
5'-CCGG-CCATTGATCAAGACAAGTTTA-CTCGAG-TAAACTTGTCTTGATCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGATCAAGACAAGTTTACTCGAGTAAACTTGTCTTGATCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGATCAAGACAAGTTTACTCGAGTAAACTTGTCTTGATCAATGG-3'

Other clones with same target sequence:

  1. TRCN0000335165