Construct: shRNA TRCN0000103400
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_009269.2-2275s1c1
- DNA Barcode:
- GCGAGCGTGTTGAGTGATATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sptlc1 (268656)
- Gene Description:
- serine palmitoyltransferase, long chain base subunit 1
- Transcript:
- RefSeq NM_009269.2 (CURRENT)
- Match location:
- Position 2275 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000103400)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGAGCGTGTTGAGTGATATA
- Hairpin Sequence:
-
5'-CCGG-GCGAGCGTGTTGAGTGATATA-CTCGAG-TATATCACTCAACACGCTCGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGAGCGTGTTGAGTGATATACTCGAGTATATCACTCAACACGCTCGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGAGCGTGTTGAGTGATATACTCGAGTATATCACTCAACACGCTCGC-3'
Other clones with same target sequence:
-
TRCN0000288225
