Construct: shRNA TRCN0000103443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010361.1-306s1c1
DNA Barcode:
CGACAACATCCGTGGTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gstt2 (14872)
Gene Description:
glutathione S-transferase, theta 2
Transcript:
RefSeq NM_010361.1 (NON-CURRENT)
Match location:
Position 306 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAACATCCGTGGTACTTT
Hairpin Sequence:
5'-CCGG-CGACAACATCCGTGGTACTTT-CTCGAG-AAAGTACCACGGATGTTGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAACATCCGTGGTACTTTCTCGAGAAAGTACCACGGATGTTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAACATCCGTGGTACTTTCTCGAGAAAGTACCACGGATGTTGTCG-3'

Other clones with same target sequence:

(none)