Construct: shRNA TRCN0000103465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182927.2-1595s1c1
DNA Barcode:
CCCACTGAGAAGACTTAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spred3 (101809)
Gene Description:
sprouty-related, EVH1 domain containing 3
Transcript:
RefSeq NM_182927.2 (NON-CURRENT)
Match location:
Position 1595 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTGAGAAGACTTAGAAT
Hairpin Sequence:
5'-CCGG-CCCACTGAGAAGACTTAGAAT-CTCGAG-ATTCTAAGTCTTCTCAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTGAGAAGACTTAGAATCTCGAGATTCTAAGTCTTCTCAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTGAGAAGACTTAGAATCTCGAGATTCTAAGTCTTCTCAGTGGG-3'

Other clones with same target sequence:

(none)