Construct: shRNA TRCN0000103532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008032.1-2559s1c1
DNA Barcode:
GCCACCTTGCATTACTTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aff2 (14266)
Gene Description:
AF4/FMR2 family, member 2
Transcript:
RefSeq NM_008032.1 (NON-CURRENT)
Match location:
Position 2559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTTGCATTACTTCTAA
Hairpin Sequence:
5'-CCGG-GCCACCTTGCATTACTTCTAA-CTCGAG-TTAGAAGTAATGCAAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTTGCATTACTTCTAACTCGAGTTAGAAGTAATGCAAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTTGCATTACTTCTAACTCGAGTTAGAAGTAATGCAAGGTGGC-3'

Other clones with same target sequence:

(none)