Construct: shRNA TRCN0000103578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008986.2-1084s1c1
DNA Barcode:
GCGCTCCAAGACCGCTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cavin1 (19285)
Gene Description:
caveolae associated 1
Transcript:
RefSeq NM_008986.2 (CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTCCAAGACCGCTGTCTA
Hairpin Sequence:
5'-CCGG-GCGCTCCAAGACCGCTGTCTA-CTCGAG-TAGACAGCGGTCTTGGAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTCCAAGACCGCTGTCTACTCGAGTAGACAGCGGTCTTGGAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTCCAAGACCGCTGTCTACTCGAGTAGACAGCGGTCTTGGAGCGC-3'

Other clones with same target sequence:

(none)