Construct: shRNA TRCN0000103601

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181324.2-563s1c1
DNA Barcode:
CCAAAGGATCTAAGAATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx6 (13209)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Transcript:
RefSeq NM_181324.2 (NON-CURRENT)
Match location:
Position 563 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103601)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGGATCTAAGAATCAAA
Hairpin Sequence:
5'-CCGG-CCAAAGGATCTAAGAATCAAA-CTCGAG-TTTGATTCTTAGATCCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGGATCTAAGAATCAAACTCGAGTTTGATTCTTAGATCCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGGATCTAAGAATCAAACTCGAGTTTGATTCTTAGATCCTTTGG-3'

Other clones with same target sequence:

(none)