Construct: shRNA TRCN0000103622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153089.2-2068s1c1
DNA Barcode:
CCTTGATTGGAGGCAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r16b (228852)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 16B
Transcript:
RefSeq NM_153089.2 (NON-CURRENT)
Match location:
Position 2068 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGATTGGAGGCAGAACTT
Hairpin Sequence:
5'-CCGG-CCTTGATTGGAGGCAGAACTT-CTCGAG-AAGTTCTGCCTCCAATCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGATTGGAGGCAGAACTTCTCGAGAAGTTCTGCCTCCAATCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGATTGGAGGCAGAACTTCTCGAGAAGTTCTGCCTCCAATCAAGG-3'

Other clones with same target sequence:

(none)