Construct: shRNA TRCN0000103626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008225.1-716s1c1
DNA Barcode:
CCACGGCGTATAAGAAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hcls1 (15163)
Gene Description:
hematopoietic cell specific Lyn substrate 1
Transcript:
RefSeq NM_008225.1 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGGCGTATAAGAAGACAA
Hairpin Sequence:
5'-CCGG-CCACGGCGTATAAGAAGACAA-CTCGAG-TTGTCTTCTTATACGCCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGGCGTATAAGAAGACAACTCGAGTTGTCTTCTTATACGCCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGGCGTATAAGAAGACAACTCGAGTTGTCTTCTTATACGCCGTGG-3'

Other clones with same target sequence:

(none)