Construct: shRNA TRCN0000103632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025599.1-427s1c1
DNA Barcode:
CGTTCCGTGATAGAATTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cmss1 (66497)
Gene Description:
cms small ribosomal subunit 1
Transcript:
RefSeq NM_025599.1 (NON-CURRENT)
Match location:
Position 427 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCCGTGATAGAATTAGAA
Hairpin Sequence:
5'-CCGG-CGTTCCGTGATAGAATTAGAA-CTCGAG-TTCTAATTCTATCACGGAACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCCGTGATAGAATTAGAACTCGAGTTCTAATTCTATCACGGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCCGTGATAGAATTAGAACTCGAGTTCTAATTCTATCACGGAACG-3'

Other clones with same target sequence:

(none)