Construct: shRNA TRCN0000103649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027835.1-3265s1c1
DNA Barcode:
CCTGATCTTGACTACTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ifih1 (71586)
Gene Description:
interferon induced with helicase C domain 1
Transcript:
RefSeq NM_027835.1 (NON-CURRENT)
Match location:
Position 3265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATCTTGACTACTCAGAA
Hairpin Sequence:
5'-CCGG-CCTGATCTTGACTACTCAGAA-CTCGAG-TTCTGAGTAGTCAAGATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATCTTGACTACTCAGAACTCGAGTTCTGAGTAGTCAAGATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATCTTGACTACTCAGAACTCGAGTTCTGAGTAGTCAAGATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000335035