Construct: shRNA TRCN0000103677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023626.3-1183s1c1
DNA Barcode:
GATTGGACTTACGACCCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ing3 (71777)
Gene Description:
inhibitor of growth family, member 3
Transcript:
RefSeq NM_023626.3 (NON-CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGGACTTACGACCCAAAT
Hairpin Sequence:
5'-CCGG-GATTGGACTTACGACCCAAAT-CTCGAG-ATTTGGGTCGTAAGTCCAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGGACTTACGACCCAAATCTCGAGATTTGGGTCGTAAGTCCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGGACTTACGACCCAAATCTCGAGATTTGGGTCGTAAGTCCAATC-3'

Other clones with same target sequence:

  1. TRCN0000381389
  2. TRCN0000317586