Construct: shRNA TRCN0000103752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010028.1-1697s1c1
DNA Barcode:
CCACCTCATTCTTTAATGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx3x (13205)
Gene Description:
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
Transcript:
RefSeq NM_010028.1 (NON-CURRENT)
Match location:
Position 1697 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTCATTCTTTAATGAAA
Hairpin Sequence:
5'-CCGG-CCACCTCATTCTTTAATGAAA-CTCGAG-TTTCATTAAAGAATGAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTCATTCTTTAATGAAACTCGAGTTTCATTAAAGAATGAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTCATTCTTTAATGAAACTCGAGTTTCATTAAAGAATGAGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000287238