Construct: shRNA TRCN0000103791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007926.1-634s1c1
DNA Barcode:
CTGGTGAATCATGTTCCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aimp1 (13722)
Gene Description:
aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
Transcript:
RefSeq NM_007926.1 (NON-CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGTGAATCATGTTCCTCTA
Hairpin Sequence:
5'-CCGG-CTGGTGAATCATGTTCCTCTA-CTCGAG-TAGAGGAACATGATTCACCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGTGAATCATGTTCCTCTACTCGAGTAGAGGAACATGATTCACCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGTGAATCATGTTCCTCTACTCGAGTAGAGGAACATGATTCACCAG-3'

Other clones with same target sequence:

  1. TRCN0000302703