Construct: shRNA TRCN0000103804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153502.2-67s1c1
DNA Barcode:
GATAGGAAAGCGTTGGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd23 (78321)
Gene Description:
ankyrin repeat domain 23
Transcript:
RefSeq NM_153502.2 (NON-CURRENT)
Match location:
Position 67 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATAGGAAAGCGTTGGAGTTT
Hairpin Sequence:
5'-CCGG-GATAGGAAAGCGTTGGAGTTT-CTCGAG-AAACTCCAACGCTTTCCTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATAGGAAAGCGTTGGAGTTTCTCGAGAAACTCCAACGCTTTCCTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATAGGAAAGCGTTGGAGTTTCTCGAGAAACTCCAACGCTTTCCTATC-3'

Other clones with same target sequence:

(none)