Construct: shRNA TRCN0000103839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178052.2-152s1c1
DNA Barcode:
GAGTTCTAATGATGACCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx59 (67997)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
Transcript:
RefSeq NM_178052.2 (NON-CURRENT)
Match location:
Position 152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCTAATGATGACCTCAA
Hairpin Sequence:
5'-CCGG-GAGTTCTAATGATGACCTCAA-CTCGAG-TTGAGGTCATCATTAGAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCTAATGATGACCTCAACTCGAGTTGAGGTCATCATTAGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCTAATGATGACCTCAACTCGAGTTGAGGTCATCATTAGAACTC-3'

Other clones with same target sequence:

(none)