Construct: shRNA TRCN0000103873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145552.1-1525s1c1
DNA Barcode:
CCATTGGAAGTTCCCACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnl2 (230737)
Gene Description:
guanine nucleotide binding protein-like 2 (nucleolar)
Transcript:
RefSeq NM_145552.1 (NON-CURRENT)
Match location:
Position 1525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGGAAGTTCCCACAGAA
Hairpin Sequence:
5'-CCGG-CCATTGGAAGTTCCCACAGAA-CTCGAG-TTCTGTGGGAACTTCCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGGAAGTTCCCACAGAACTCGAGTTCTGTGGGAACTTCCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGGAAGTTCCCACAGAACTCGAGTTCTGTGGGAACTTCCAATGG-3'

Other clones with same target sequence:

(none)