Construct: shRNA TRCN0000103935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_284530.1-2259s1c1
DNA Barcode:
GCTGTTGTTGTGCATACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd34c (330998)
Gene Description:
ankyrin repeat domain 34C
Transcript:
RefSeq XM_284530.1 (NON-CURRENT)
Match location:
Position 2259 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGTTGTGCATACTAAA
Hairpin Sequence:
5'-CCGG-GCTGTTGTTGTGCATACTAAA-CTCGAG-TTTAGTATGCACAACAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGTTGTGCATACTAAACTCGAGTTTAGTATGCACAACAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGTTGTGCATACTAAACTCGAGTTTAGTATGCACAACAACAGC-3'

Other clones with same target sequence:

(none)