Construct: shRNA TRCN0000103955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080856.1-837s1c1
DNA Barcode:
GCGCTACAATACACATTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asb14 (142687)
Gene Description:
ankyrin repeat and SOCS box-containing 14
Transcript:
RefSeq NM_080856.1 (NON-CURRENT)
Match location:
Position 837 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTACAATACACATTGAAA
Hairpin Sequence:
5'-CCGG-GCGCTACAATACACATTGAAA-CTCGAG-TTTCAATGTGTATTGTAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTACAATACACATTGAAACTCGAGTTTCAATGTGTATTGTAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTACAATACACATTGAAACTCGAGTTTCAATGTGTATTGTAGCGC-3'

Other clones with same target sequence:

(none)