Construct: shRNA TRCN0000103985

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004364.1-4399s1c1
DNA Barcode:
CGCATCTGTATCATCGGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asap2 (211914)
Gene Description:
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Transcript:
RefSeq NM_001004364.1 (NON-CURRENT)
Match location:
Position 4399 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000103985)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATCTGTATCATCGGTCTT
Hairpin Sequence:
5'-CCGG-CGCATCTGTATCATCGGTCTT-CTCGAG-AAGACCGATGATACAGATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATCTGTATCATCGGTCTTCTCGAGAAGACCGATGATACAGATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATCTGTATCATCGGTCTTCTCGAGAAGACCGATGATACAGATGCG-3'

Other clones with same target sequence:

(none)