Construct: shRNA TRCN0000104019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053183.1-2051s1c1
DNA Barcode:
CCAGCCAAATTACCTGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx50 (94213)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Transcript:
RefSeq NM_053183.1 (NON-CURRENT)
Match location:
Position 2051 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCAAATTACCTGAAATT
Hairpin Sequence:
5'-CCGG-CCAGCCAAATTACCTGAAATT-CTCGAG-AATTTCAGGTAATTTGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCAAATTACCTGAAATTCTCGAGAATTTCAGGTAATTTGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCAAATTACCTGAAATTCTCGAGAATTTCAGGTAATTTGGCTGG-3'

Other clones with same target sequence:

(none)