Construct: shRNA TRCN0000104030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028074.2-3058s1c1
DNA Barcode:
GCGTCGTCAGTCGTCTTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx42 (72047)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Transcript:
RefSeq NM_028074.2 (NON-CURRENT)
Match location:
Position 3058 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCGTCAGTCGTCTTTAAT
Hairpin Sequence:
5'-CCGG-GCGTCGTCAGTCGTCTTTAAT-CTCGAG-ATTAAAGACGACTGACGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCGTCAGTCGTCTTTAATCTCGAGATTAAAGACGACTGACGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCGTCAGTCGTCTTTAATCTCGAGATTAAAGACGACTGACGACGC-3'

Other clones with same target sequence:

  1. TRCN0000309020