Construct: shRNA TRCN0000104062
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020494.2-2532s1c1
- DNA Barcode:
- GCCCTAGAGATTGAGCTAGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ddx24 (27225)
- Gene Description:
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
- Transcript:
- RefSeq NM_020494.2 (NON-CURRENT)
- Match location:
- Position 2532 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000104062)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCTAGAGATTGAGCTAGAA
- Hairpin Sequence:
-
5'-CCGG-GCCCTAGAGATTGAGCTAGAA-CTCGAG-TTCTAGCTCAATCTCTAGGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCTAGAGATTGAGCTAGAACTCGAGTTCTAGCTCAATCTCTAGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCTAGAGATTGAGCTAGAACTCGAGTTCTAGCTCAATCTCTAGGGC-3'
Other clones with same target sequence:
-
TRCN0000317020
