Construct: shRNA TRCN0000104279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009079.2-326s1c1
DNA Barcode:
CGCCAACAGCAAAGAGAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpl22 (19934)
Gene Description:
ribosomal protein L22
Transcript:
RefSeq NM_009079.2 (NON-CURRENT)
Match location:
Position 326 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAACAGCAAAGAGAGTTA
Hairpin Sequence:
5'-CCGG-CGCCAACAGCAAAGAGAGTTA-CTCGAG-TAACTCTCTTTGCTGTTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAACAGCAAAGAGAGTTACTCGAGTAACTCTCTTTGCTGTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAACAGCAAAGAGAGTTACTCGAGTAACTCTCTTTGCTGTTGGCG-3'

Other clones with same target sequence:

  1. TRCN0000353825