Construct: shRNA TRCN0000104530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053159.2-1364s1c1
DNA Barcode:
GCATTAAACAAACAGTCGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrpl3 (94062)
Gene Description:
mitochondrial ribosomal protein L3
Transcript:
RefSeq NM_053159.2 (NON-CURRENT)
Match location:
Position 1364 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTAAACAAACAGTCGCAT
Hairpin Sequence:
5'-CCGG-GCATTAAACAAACAGTCGCAT-CTCGAG-ATGCGACTGTTTGTTTAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTAAACAAACAGTCGCATCTCGAGATGCGACTGTTTGTTTAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTAAACAAACAGTCGCATCTCGAGATGCGACTGTTTGTTTAATGC-3'

Other clones with same target sequence:

(none)