Construct: shRNA TRCN0000104561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025877.3-458s1c1
DNA Barcode:
CGGAACCAAGATGGTCACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a23 (66972)
Gene Description:
solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
Transcript:
RefSeq NM_025877.3 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACCAAGATGGTCACATA
Hairpin Sequence:
5'-CCGG-CGGAACCAAGATGGTCACATA-CTCGAG-TATGTGACCATCTTGGTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACCAAGATGGTCACATACTCGAGTATGTGACCATCTTGGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACCAAGATGGTCACATACTCGAGTATGTGACCATCTTGGTTCCG-3'

Other clones with same target sequence:

(none)