Construct: shRNA TRCN0000104669

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177911.2-550s1c1
DNA Barcode:
CGCCAAACAAATTAAAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgm4 (331046)
Gene Description:
transglutaminase 4 (prostate)
Transcript:
RefSeq NM_177911.2 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104669)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAAACAAATTAAAGAGAA
Hairpin Sequence:
5'-CCGG-CGCCAAACAAATTAAAGAGAA-CTCGAG-TTCTCTTTAATTTGTTTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAAACAAATTAAAGAGAACTCGAGTTCTCTTTAATTTGTTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAAACAAATTAAAGAGAACTCGAGTTCTCTTTAATTTGTTTGGCG-3'

Other clones with same target sequence:

(none)