Construct: shRNA TRCN0000104680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028643.1-1829s1c1
DNA Barcode:
CCTGCCTTGAAATAGCACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Micu2 (68514)
Gene Description:
mitochondrial calcium uptake 2
Transcript:
RefSeq NM_028643.1 (NON-CURRENT)
Match location:
Position 1829 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCTTGAAATAGCACATA
Hairpin Sequence:
5'-CCGG-CCTGCCTTGAAATAGCACATA-CTCGAG-TATGTGCTATTTCAAGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCTTGAAATAGCACATACTCGAGTATGTGCTATTTCAAGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCTTGAAATAGCACATACTCGAGTATGTGCTATTTCAAGGCAGG-3'

Other clones with same target sequence:

(none)