Construct: shRNA TRCN0000104731
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_174998.1-305s1c1
- DNA Barcode:
- CAGGAGCTAAAGCAGTGGTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hpcal4 (170638)
- Gene Description:
- hippocalcin-like 4
- Transcript:
- RefSeq NM_174998.1 (NON-CURRENT)
- Match location:
- Position 305 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000104731)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGGAGCTAAAGCAGTGGTAT
- Hairpin Sequence:
-
5'-CCGG-CAGGAGCTAAAGCAGTGGTAT-CTCGAG-ATACCACTGCTTTAGCTCCTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGGAGCTAAAGCAGTGGTATCTCGAGATACCACTGCTTTAGCTCCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGGAGCTAAAGCAGTGGTATCTCGAGATACCACTGCTTTAGCTCCTG-3'
Other clones with same target sequence:
(none)
