Construct: shRNA TRCN0000104731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174998.1-305s1c1
DNA Barcode:
CAGGAGCTAAAGCAGTGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpcal4 (170638)
Gene Description:
hippocalcin-like 4
Transcript:
RefSeq NM_174998.1 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGCTAAAGCAGTGGTAT
Hairpin Sequence:
5'-CCGG-CAGGAGCTAAAGCAGTGGTAT-CTCGAG-ATACCACTGCTTTAGCTCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGCTAAAGCAGTGGTATCTCGAGATACCACTGCTTTAGCTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGCTAAAGCAGTGGTATCTCGAGATACCACTGCTTTAGCTCCTG-3'

Other clones with same target sequence:

(none)