Construct: shRNA TRCN0000104739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144525.2-1318s1c1
DNA Barcode:
CACGGAAATCTTTACAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem214 (68796)
Gene Description:
transmembrane protein 214
Transcript:
RefSeq NM_144525.2 (NON-CURRENT)
Match location:
Position 1318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGGAAATCTTTACAAGAAA
Hairpin Sequence:
5'-CCGG-CACGGAAATCTTTACAAGAAA-CTCGAG-TTTCTTGTAAAGATTTCCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGGAAATCTTTACAAGAAACTCGAGTTTCTTGTAAAGATTTCCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGGAAATCTTTACAAGAAACTCGAGTTTCTTGTAAAGATTTCCGTG-3'

Other clones with same target sequence:

  1. TRCN0000327278