Construct: shRNA TRCN0000104857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030260.1-1149s1c1
DNA Barcode:
CCTGGGTGCAATAAACAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zxdc (80292)
Gene Description:
ZXD family zinc finger C
Transcript:
RefSeq NM_030260.1 (NON-CURRENT)
Match location:
Position 1149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGTGCAATAAACAATAT
Hairpin Sequence:
5'-CCGG-CCTGGGTGCAATAAACAATAT-CTCGAG-ATATTGTTTATTGCACCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGTGCAATAAACAATATCTCGAGATATTGTTTATTGCACCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGTGCAATAAACAATATCTCGAGATATTGTTTATTGCACCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000302898