Construct: shRNA TRCN0000104883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009494.1-167s1c1
DNA Barcode:
TGGAGGATAAAGAATAGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn2r-ps159 (22314)
Gene Description:
vomeronasal 2, receptor, pseudogene 159
Transcript:
RefSeq NM_009494.1 (NON-CURRENT)
Match location:
Position 167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGGATAAAGAATAGTGAT
Hairpin Sequence:
5'-CCGG-TGGAGGATAAAGAATAGTGAT-CTCGAG-ATCACTATTCTTTATCCTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGGATAAAGAATAGTGATCTCGAGATCACTATTCTTTATCCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGGATAAAGAATAGTGATCTCGAGATCACTATTCTTTATCCTCCA-3'

Other clones with same target sequence:

(none)