Construct: shRNA TRCN0000104898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013385.1-694s1c1
DNA Barcode:
CTTGAGTGATAACAGCCGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm4 (268934)
Gene Description:
glutamate receptor, metabotropic 4
Transcript:
RefSeq NM_001013385.1 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAGTGATAACAGCCGCTA
Hairpin Sequence:
5'-CCGG-CTTGAGTGATAACAGCCGCTA-CTCGAG-TAGCGGCTGTTATCACTCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAGTGATAACAGCCGCTACTCGAGTAGCGGCTGTTATCACTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAGTGATAACAGCCGCTACTCGAGTAGCGGCTGTTATCACTCAAG-3'

Other clones with same target sequence:

(none)