Construct: shRNA TRCN0000104937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010942.2-386s1c1
DNA Barcode:
CCTGGTTGTCTACAAAGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nsg1 (18196)
Gene Description:
neuron specific gene family member 1
Transcript:
RefSeq NM_010942.2 (NON-CURRENT)
Match location:
Position 386 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTTGTCTACAAAGTGTA
Hairpin Sequence:
5'-CCGG-CCTGGTTGTCTACAAAGTGTA-CTCGAG-TACACTTTGTAGACAACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTTGTCTACAAAGTGTACTCGAGTACACTTTGTAGACAACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTTGTCTACAAAGTGTACTCGAGTACACTTTGTAGACAACCAGG-3'

Other clones with same target sequence:

  1. TRCN0000317908