Construct: shRNA TRCN0000104970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139227.2-3135s1c1
DNA Barcode:
CGTGAGAAGTTTGTGTTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atxn7 (246103)
Gene Description:
ataxin 7
Transcript:
RefSeq NM_139227.2 (NON-CURRENT)
Match location:
Position 3135 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000104970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAGAAGTTTGTGTTTGTA
Hairpin Sequence:
5'-CCGG-CGTGAGAAGTTTGTGTTTGTA-CTCGAG-TACAAACACAAACTTCTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAGAAGTTTGTGTTTGTACTCGAGTACAAACACAAACTTCTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAGAAGTTTGTGTTTGTACTCGAGTACAAACACAAACTTCTCACG-3'

Other clones with same target sequence:

(none)