Construct: shRNA TRCN0000105096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145525.2-2172s1c1
DNA Barcode:
CCGACAACATTTCTCGGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Osbpl6 (99031)
Gene Description:
oxysterol binding protein-like 6
Transcript:
RefSeq NM_145525.2 (NON-CURRENT)
Match location:
Position 2172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACAACATTTCTCGGCAAA
Hairpin Sequence:
5'-CCGG-CCGACAACATTTCTCGGCAAA-CTCGAG-TTTGCCGAGAAATGTTGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACAACATTTCTCGGCAAACTCGAGTTTGCCGAGAAATGTTGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACAACATTTCTCGGCAAACTCGAGTTTGCCGAGAAATGTTGTCGG-3'

Other clones with same target sequence:

(none)