Construct: shRNA TRCN0000105115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152818.2-3144s1c1
DNA Barcode:
GCCACATAACTATCCTGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Osbp2 (74309)
Gene Description:
oxysterol binding protein 2
Transcript:
RefSeq NM_152818.2 (CURRENT)
Match location:
Position 3144 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATAACTATCCTGGCAA
Hairpin Sequence:
5'-CCGG-GCCACATAACTATCCTGGCAA-CTCGAG-TTGCCAGGATAGTTATGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATAACTATCCTGGCAACTCGAGTTGCCAGGATAGTTATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATAACTATCCTGGCAACTCGAGTTGCCAGGATAGTTATGTGGC-3'

Other clones with same target sequence:

(none)