Construct: shRNA TRCN0000105131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_135054.4-690s1c1
DNA Barcode:
CCAGTGGGTTATGTTATTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700057G04Rik (78459)
Gene Description:
RIKEN cDNA 1700057G04 gene
Transcript:
RefSeq XM_135054.4 (NON-CURRENT)
Match location:
Position 690 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGGGTTATGTTATTCAA
Hairpin Sequence:
5'-CCGG-CCAGTGGGTTATGTTATTCAA-CTCGAG-TTGAATAACATAACCCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGGGTTATGTTATTCAACTCGAGTTGAATAACATAACCCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGGGTTATGTTATTCAACTCGAGTTGAATAACATAACCCACTGG-3'

Other clones with same target sequence:

(none)