Construct: shRNA TRCN0000105145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175391.2-1672s1c1
DNA Barcode:
CCTCATTGTGTGTTTATCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apol7c (108956)
Gene Description:
apolipoprotein L 7c
Transcript:
RefSeq NM_175391.2 (NON-CURRENT)
Match location:
Position 1672 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTGTGTGTTTATCCAA
Hairpin Sequence:
5'-CCGG-CCTCATTGTGTGTTTATCCAA-CTCGAG-TTGGATAAACACACAATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTGTGTGTTTATCCAACTCGAGTTGGATAAACACACAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTGTGTGTTTATCCAACTCGAGTTGGATAAACACACAATGAGG-3'

Other clones with same target sequence:

(none)