Construct: shRNA TRCN0000105155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029419.1-2177s1c1
DNA Barcode:
CCACTGTGGATGTTAAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apol7a (75761)
Gene Description:
apolipoprotein L 7a
Transcript:
RefSeq NM_029419.1 (NON-CURRENT)
Match location:
Position 2177 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGTGGATGTTAAGGAAA
Hairpin Sequence:
5'-CCGG-CCACTGTGGATGTTAAGGAAA-CTCGAG-TTTCCTTAACATCCACAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGTGGATGTTAAGGAAACTCGAGTTTCCTTAACATCCACAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGTGGATGTTAAGGAAACTCGAGTTTCCTTAACATCCACAGTGG-3'

Other clones with same target sequence:

(none)